Metarhizium spp. isolates effective against Queensland fruit fly juvenile life stages in soil.

Queensland fruit fly, Bactrocera tryoni, Froggatt (Diptera: Tephritidae) is Australia's primary fruit fly pest species. Integrated Pest Management (IPM) has been adopted to sustainably manage this polyphagous species with a reduced reliance on chemical pesticides. At present, control measures are aimed at the adult stages of the fly, with no IPM tools available to target larvae once they exit the fruit and pupate in the soil. The use of entomopathogenic fungi may provide a biologically-based control method for these soil-dwelling life stages. The effectiveness of fungal isolates of Metarhizium and Beauveria species were screened under laboratory conditions against Queensland fruit fly. In bioassays, 16 isolates were screened for pathogenicity following exposure of third-instar larvae to inoculum-treated vermiculite used as a pupation substrate. The best performing Metarhizium sp. isolate achieved an average percentage mortality of 93%, whereas the best performing Beauveria isolate was less efficient, with an average mortality of 36%. Susceptibility to infection during different development stages was investigated using selected fungal isolates, with the aim of assessing all soil-dwelling life stages from third-instar larvae to final pupal stages and emerging adults. Overall, the third larval instar was the most susceptible stage, with average mortalities between 51-98% depending on the isolate tested. Moreover, adult mortality was significantly higher when exposed to inoculum during pupal eclosion, with mortalities between 56-76% observed within the first nine days post-emergence. The effect of temperature and inoculum concentration on insect mortality were assessed independently with candidate isolates to determine the optimum temperature range for fungal biological control activity and the rate required for application in field conditions. Metarhizium spp. are highly efficacious at killing Queensland fruit fly and have potential for use as biopesticides to target soil-dwelling and other life stages of B. tryoni.

Precision Monitoring of Honey Bee (Hymenoptera: Apidae) Activity and Pollen Diversity during Pollination to Evaluate Colony Health.

Certain crops depend upon pollination services for fruit set, and, of these, almonds are of high value for Australia. Stressors, such as diseases, parasites, pesticides, and nutrition, can contribute to honey bee Apis mellifera L. colony decline, thereby reducing bee activity and pollination efficiency. In Australia, field studies are required to monitor honey bee health and to ascertain whether factors associated with colony decline are impacting hives. We monitored honey bee colonies during and after pollination services of almond. Video surveillance technology was used to quantify bee activity, and bee-collected pollen was periodically tested for pesticide residues. Plant species diversity was also assessed using DNA metabarcoding of the pollen. Results showed that bee activity increased in almond but not in bushland. Residues detected included four fungicides, although the quantities were of low risk of oral toxicity to bees. Floral diversity was lower in the pollen collected by bees from almonds compared to bushland. However, diversity was higher at the onset and conclusion of the almond bloom, suggesting that bees foraged more widely when availability was low. Our findings suggest that commercial almond orchards may sustain healthier bee colonies compared to bushland in early spring, although the magnitude of the benefit is likely landscape-dependent.

A reduction in the pupil's response to affective sounds in psychopathy and related personality traits.

The pupil of the eye dilates in response to affective information, even if that information is not visual. We used this affective modulation of the pupil to examine the hypothesis that individuals with high traits of psychopathy have an insensitivity to emotional stimuli. We also examined general personality traits related to psychopathy. A sample of 120 healthy young men had their pupils monitored while they listened to sound clips that conveyed either neutral emotion (e.g., rain), negative emotion (e.g., a person screaming) or positive emotion (e.g., people laughing). Psychopathy and general personality traits were measured via self-report questionnaire. As expected, both the positive and negative emotional sounds produced greater dilation in the pupil size than neutral sounds. This affective modulation of the pupil was found to be reduced for the negative sounds for people high on the "callous/affective" components of psychopathy (the Affective facet of the SRP-4 and the Meanness scale of the TriPM) and the general personality traits of Reward Dependence and Cooperativeness. The results indicate that these callous traits of psychopathy and general personality may be underpinned by a reduction in the ability to effectively process or monitor emotional stimuli.

Automatic processing of emotional images and psychopathic personality traits.

Psychopathy is associated with a deficit in affective processes and might be reflected in the inability to extract the emotional content of a stimulus. Across two experiments, we measured the interference effect from emotional images that were irrelevant to the processing of simultaneous target stimuli and examined if this interference was moderated by psychometrically defined traits of psychopathy. In Experiment 1, we showed this emotional distraction effect was reduced as a function of psychopathic traits related to cold-heartedness and occurred for both positively- and negatively-valenced images. Experiment 2 attempted to test the automaticity of the effects by presenting the emotional stimuli briefly so that the emotion was difficult to report. Again, high visibility images produced strong effects that were moderated by the cold-heatedness/meanness traits of psychopathy, but the low-visibility images did not evoke the emotional distractor effect. Our results strongly support the notion that psychopathic traits related to cold-heartedness/meanness are associated with an inability to automatically process the emotional content of images.

Post-traumatic stress disorder and major depression among frontline healthcare staff working during the COVID-19 pandemic.

OBJECTIVES: High rates of probable post-traumatic stress disorder (PTSD) and major depressive disorder (MDD) have been reported for frontline healthcare staff during the COVID-19 pandemic. However, rates determined by diagnostic assessment are unknown, as are the onset of symptoms and associated index events. METHODS: We assessed frontline healthcare staff with the Structured Clinical Interview for DSM-5. RESULTS: Forty-four percent met criteria for PTSD and 39% met criteria for MDD. Twenty-four percent reported COVID-19 trauma as their index event, with the majority of staff reporting trauma that pre-dated the pandemic. While PTSD was likely to be pre-existing, MDD was more likely to develop during pandemic working. CONCLUSION: These findings indicate the propensity of healthcare staff to experience a range of occupational and personal trauma associated with PTSD and the need to assess index trauma when diagnosing psychopathology in order to best understand the needs of this workforce. PRACTITIONER POINTS: We found high diagnostic rates of PTSD (44%) and major depression (39%) among frontline healthcare staff working during the COVID-19 pandemic. Although major depression developed during the pandemic, PTSD was more likely to be pre-existing. When assessing pandemic-related psychopathology, it is important to assess the onset and index event related to symptoms. Healthcare workers appear to have high rates of PTSD related to occupational and personal trauma, which warrants specific focus in service planning.

Abstract thinking as a risk factor for the development of posttraumatic stress disorder symptoms in student paramedics.

cognitive processing is characterized by "why?" and "what if?" questions and associated with processes such as rumination and worry. The tendency to think abstractly in response to stress has not been examined as a longitudinal risk factor for later stress reactions. The present study evaluated the extent to which an abstract thinking style could represent a risk factor for posttraumatic stress disorder (PTSD) symptoms in student paramedics over a 6-month follow-up period. Student paramedics (N = 89) recruited from universities in the United Kingdom were assessed for baseline symptoms, abstract thinking, and cognitive responses to stressful memories; a follow-up assesment was conducted 6 months later in their training. All participants were exposed to a potentially traumatic event between baseline and follow-up. Baseline symptoms and trauma history accounted for 45% of the variation in follow-up posttraumatic symptoms, with abstract thinking style explaining an additional 2.5% over and above what could be predicted from initial symptom levels. Abstract thinking was moderately related to rumination in response to stressful memories, r = .45, and correlated with follow-up symptoms of PTSD, r = .49; anxiety, r = .40; and depression, r = .27. This study builds on previous work suggesting that abstract processing mode and abstract rumination are risk factors for PTSD. Abstract thinking in response to stress represents a potentially modifiable risk factor that could be targeted by a resilience-focused intervention for individuals likely to encounter traumatic events.

Structured Professional Judgment to Assist the Evaluation and Safety Planning of Suicide Risk: The Risk of Suicide Protocol (RoSP).

Background: The Risk of Suicide Protocol (RoSP) is a structured professional judgment (SPJ) scheme designed in line with NICE guidelines to improve clinicians' ability to evaluate and manage suicide risk. Aims: This study aimed to evaluate the efficacy of RoSP in two settings: (1) unexpected deaths of people in the community who were known to mental health services; and (2) an inpatient hospital specializing in the assessment and treatment of patients with personality disorder. Method: In Study 1, information from a database of unexpected deaths (N = 68) within an NHS health board was used to complete a RoSP assessment (blind to cause of death) and information from the Coroner's Court was used to assign people to suicide vs. natural causes/accidental death. In Study 2, patients (N = 62) were assessed on the RoSP upon admission to hospital and their self-injurious behaviors were recorded over the first 3 months of admission. Results: (1) Evaluations using RoSP were highly reliable in both samples (ICCs 0.93-0.98); (2) professional judgment based on the RoSP was predictive of completed suicide in the community sample (AUC = 0.83) and; (3) was predictive of both suicide attempts (AUC = 0.81) and all self-injurious behaviors (AUC = 0.80) for the inpatient sample. Conclusion: RoSP is a reliable and valid instrument for the structured clinical evaluation of suicide risk for use in inpatient psychiatric services and in community mental health services. RoSP's efficacy is comparable to well-established structured professional judgment instruments designed to predict other risk behavior (e.g., HCR-20 and the prediction of violence). The use of RoSP for the clinical evaluation of suicide risk and safety-planning provides a structure for meeting NICE guidelines for suicide prevention and is now evidence-based.

Mitochondrial evolution in the entomopathogenic fungal genus Beauveria.

Species in the fungal genus Beauveria are pathogens of invertebrates and have been commonly used as the active agent in biopesticides. After many decades with few species described, recent molecular approaches to classification have led to over 25 species now delimited. Little attention has been given to the mitochondrial genomes of Beauveria but better understanding may led to insights into the nature of species and evolution in this important genus. In this study, we sequenced the mitochondrial genomes of four new strains belonging to Beauveria bassiana, Beauveria caledonica and Beauveria malawiensis, and compared them to existing mitochondrial sequences of related fungi. The mitochondrial genomes of Beauveria ranged widely from 28,806 to 44,135 base pairs, with intron insertions accounting for most size variation and up to 39% (B. malawiensis) of the mitochondrial length due to introns in genes. Gene order of the common mitochondrial genes did not vary among the Beauveria sequences, but variation was observed in the number of transfer ribonucleic acid genes. Although phylogenetic analysis using whole mitochondrial genomes showed, unsurprisingly, that B. bassiana isolates were the most closely related to each other, mitochondrial codon usage suggested that some B. bassiana isolates were more similar to B. malawiensis and B. caledonica than the other B. bassiana isolates analyzed.

Brief cognitive behavioural therapy for post-stroke 'delusional infestation' in a 71-year-old man: a single case experimental design.

BACKGROUND: Delusional infestation is a condition at the interface of tactile and visual hallucinations and delusions. Individuals with this condition hold the fixed and false belief that their body or their environment is infested with parasites, insects or other organisms. AIMS: There are no guidelines or publications detailing the psychological assessment, formulation, intervention and evaluation of this presentation. This paper aims to address this gap. METHOD: Single case experimental design methodology was employed to evaluate the use of cognitive behavioural therapy (CBT) for delusional infestation in a 70-year-old male who was intolerant of anti-psychotic medication. 'Tom' had a large, mature infarct in the middle cerebral artery territory as well as a left posterior parietal infarct post-stroke, which may have precipitated his symptoms. After a baseline period of 3 weeks, Tom received eight sessions of CBT based on the model by Collerton and Dudley (2004). RESULTS: Post-intervention, there was a reliable improvement on clinical measures as well as a large reduction in distress levels, which was maintained at 3-month follow-up. The conviction in the belief that the infestation was real did not shift. CONCLUSION: This case demonstrated the potential for the use of CBT to address distress related to delusional infestation. This work is discussed in relation to post-stroke psychosis, psychological therapies with older adults, and suggestions are made for future research.

Enhanced emotional response to both negative and positive images in post-traumatic stress disorder: Evidence from pupillometry.

Post-traumatic stress disorder (PTSD) is characterised by alterations in the function of the autonomic nervous system. However, it is unclear if this dysfunction is threat-related or related to arousing stimuli in general. Pupillometry offers a simple non-invasive measure of ANS activity that can separate parasympathetic and sympathetic arousal. Participants viewed images with emotional or neutral content: 20 met diagnostic criteria for PTSD, 28 were trauma-exposed (but with no PTSD), and 17 were controls. Initial pupil constriction (a marker of parasympathetic function) was reduced for the PTSD group, while dilation due to the emotional content of the image (a marker of sympathetic activity) was greater in the PTSD group. Individuals with PTSD demonstrated enhanced physiological arousal to both threat-related and positive images. The results suggest reduced parasympathetic arousal and increased sympathetic arousal in the autonomic nervous system, which has been linked to a range of adverse health outcomes in PTSD.

Sexual Attraction to Both Genders in Ambiphilic Men: Evidence from Implicit Cognitions.

Ambiphilic (or bisexual) men describe feelings of sexual attraction to both men and women. However, physiological measures of arousal have failed to show a consistent pattern of arousal to both genders. We measured men's automatic associations between the concept of sex (represented by words) and the concepts of men versus women (represented by images) via the Implicit Association Test (IAT) and a priming task. On the IAT, gynephilic men (N = 32) were faster for women-sex pairings, androphilic men (N = 18) were faster for men-sex pairings, while ambiphilic men (N = 20) showed no bias toward either gender. We then isolated the concepts of "men" and "women" by comparing them separately against neutral images. In contrast to both the gynephilic or androphilic men, ambiphilic men showed sexual associations to both men and women. On the priming task, ambiphilic men showed faster responses to sex words, but slower responses to not-sex words, when primed with pictures of either men or women compared to when primed by neutral images. The results from all the experimental tasks suggest that ambiphilic men have a pattern of sexual association that is different from both gynephilic and androphilic men and represents a sexual attraction to both men and women.

Pupillary Responses to Static Images of Men and Women: A Possible Measure of Sexual Interest?

The pupil dilates to images that are arousing. In Experiment 1, we examined if the pupil's response to brief presentations (2,000 ms) of static images could be used to identify individuals' sexual orientation. Participants were grouped according to their self-reported gender and sexual orientation (male heterosexual, N = 20; male bisexual, N = 13; male homosexual, N = 19; female heterosexual, N = 28; female bisexual, N = 21; female homosexual, N = 17). Pupil size was monitored to images of men in seminude poses, women in seminude poses, or neutral images. Every group showed the same pattern of responses, with the greatest dilation to male images, then female images, and least dilation to the neutral images. Experiment 2 used more tightly controlled stimuli and tested at two different image durations (150 and 3,000 ms). Both heterosexual men (N = 18) and women (N = 20) showed greater pupil dilation to images of nude men than to nude women. However, in Experiment 3, where we reduced the erotic content by using images of clothed models, both heterosexual men and women showed greater pupil dilation to images of women. The results showed that while the pupil does dilate strongly to sexual imagery, its response to these brief static images does not correspond to a person's sexual orientation in a simple manner.

Detection of the Entomopathogenic Fungus Beauveria bassiana in the Rhizosphere of Wound-Stressed Zea mays Plants.

Entomopathogenic fungi from the genus Beauveria (Vuillemin) play an important role in controlling insect populations and have been increasingly utilized for the biological control of insect pests. Various studies have reported that Beauveria bassiana (Bals.), Vuill. also has the ability to colonize a broad range of plant hosts as endophytes without causing disease but while still maintaining the capacity to infect insects. Beauveria is often applied as an inundative spore application, but little research has considered how plant colonization may alter the ability to persist in the environment. The aim of this study was to investigate potential interactions between B. bassiana and Zea mays L. (maize) in the rhizosphere following inoculation, in order to understand the factors that may affect environmental persistence of the fungi. The hypothesis was that different isolates of B. bassiana have the ability to colonize maize roots and/or rhizosphere soil, resulting in effects to the plant microbiome. To test this hypothesis, a two-step nested PCR protocol was developed to find and amplify Beauveria in planta or in soil; based on the translation elongation factor 1-alpha (ef1α) gene. The nested protocol was also designed to enable Beauveria species differentiation by sequence analysis. The impact of three selected B. bassiana isolates applied topically to roots on the rhizosphere soil community structure and function were consequently assessed using denaturing gradient gel electrophoresis (DGGE) and MicroRespTM techniques. The microbial community structure and function were not significantly affected by the presence of the isolates, however, retention of the inocula in the rhizosphere at 30 days after inoculation was enhanced when plants were subjected to intensive wounding of foliage to crudely simulate herbivory. The plant defense response likely changed under wound stress resulting in the apparent recruitment of Beauveria in the rhizosphere, which may be an indirect defensive strategy against herbivory and/or the result of induced systemic susceptibility in maize enabling plant colonization.

Plant Tissue Preparation for the Detection of an Endophytic Fungus In Planta.

The identification of fungal endophytes often relies on culturing isolates from surface-sterilized plant tissue. However, molecular techniques have enabled the rapid detection and identification of targeted endophyte species, and next-generation sequencing technology provides an opportunity to obtain comprehensive information on endophytic communities, directly from plant tissue. In order to achieve accurate detection from internal tissues, surface microbes and associated deoxyribonucleic acid (DNA) must be eliminated, with particular consideration for the type of plant tissue and the efficacy of the surface sterilization procedure used. The methodology described later was developed specifically for detection of DNA from the entomopathogenic fungal endophyte Beauveria bassiana (Vuillemin) (Ascomycota: Hypocreales) in various tissues of Zea mays (L.). However, the protocol may be easily applied to other fungi and bacterial endophytes. Included is a stringent sodium hypochlorite-based surface sterilization protocol for plant material in preparation for polymerase chain reaction (PCR) to detect target DNA within plant tissue. Included are a treatment for dealing with surface DNA contamination and a novel procedure for assessing the efficacy of surface sterilization using PCR.